Determination of Atenolol in Pharmaceutical Preparation by Zero-, First-, Second- and Third- Order Derivative Spectrophotometric Methods

In this study, zero-, first-, secondand third-order derivative spectrophotometric methods were developed for quantitave determination of atenolol in pharmaceutical preparation. In zero order spectrophotometry, absorbance values were measured at 276 nm in zero order spectra of solution of atenolol in methanol in the range of 245-310 nm. In first derivative spectrophotometry, absorbance values were measured at 273, 276 and 285 nm. In second derivative spectrophotometry, absorbance values were measured at 276, 279, 282 and 287 nm. In third derivative spectrophotometry, absorbance values were measured at 275, 278 and 281 nm. Parameters such as linearity, precision, accuracy, specificity, stability, limit of detection and limit of quantitation were studied according to the International Conference on Harmonization Guidelines. Calibration curves were linear between the concentration range of 2.5-17.5 μg ml-1. Withinand between-day precision values for atenolol were less than 6.04%, and accuracy (relative error) was better than 5.12%. The mean recovery value of atenolol was 100.9% for pharmaceutical preparation. All the methods developed were successfully applied to a tablet formulation and the results were compared statistically with each other.